Comparative epigenomics of cis-regulatory elements
Understanding the establishment of GNRs requires advanced knowledge on key epigenetic modifications and chromatin-modifying enzymes involved at the promoters and enhancers of genes with dynamic developmental expression patterns. Available epigenomic data will be used to identify and compare, at a genome-wide level, all cis-regulatory elements (enhancers and promoters) that operate at different developmental stages in Xenopus and zebrafish. The activity of these cis-regulatory elements through development will be analysed via interpretation of chromatin loops and epigenetic modifications.
Figure 2. 4C-seq analysis of embryonic chromosomal interactions at the zebrafish Six2/3 loci.